It has been a pleasure to work with you... We have greatly appreciated the level of communication and customer service from MediMabs.
I had a conversation this morning with my South African colleagues who have recently tried the antibodies you produce. They have informed me that the antibodies worked beautifully for the western blots against the component vaccine strains, so I wanted to tell you about their positive comments in relation to the product of your work (translation).
Dear Emmanuelle, I would like to take this opportunity to thank you for the quality of the services you have provided me. I truly feel that yourself and your team have been very efficient and readily available for very good advice. The anti-axin monoclonal antibody that you have produced for me is of very good quality and has become an important tool in our research projects. Your assistance was very useful in the success of production of certain clones (5G5, AA5). AA5 particularly presented some difficulties.
Dear Dr. Susana Garcia-Franco, We have received the package with the hybridoma cells and the fused cells and I have placed them straight into liquid nitrogen. Just to let you know your methods and help with this project was excellent. I know it is a very difficult project we have undertaken.
Dear Emmanuelle, As you are aware, our objective is to obtain good quality monoclonal antibodies for proteins involved in cell cycle regulation. Many of the proteins we are interested in are low-abundance proteins that show large fluctuations in their expression levels during the cell cycle. As such, these proteins have been historically difficult to detect by immunoblotting or immunofluorescence without using artificial fusions with ectopic epitopes. The 4 monoclonal antibodies that you have generated for us have been working very well so far in immunoblotting experiments. They detect endogenous proteins without overexpression or fusion to tandem epitope sequences. Throughout the antibody production process, I appreciated the regular contacts I had with you and other scientists at MédiMabs, such as Drs Pierre-André Scott and Vanessa Tumilasci, to ensure that we effectively obtain the right type of antibody for our application. I am looking forward to continuing our work together in producing high-quality antibodies for difficult-to-detect cell cycle proteins.
Dear Dr Roux: I am writing to thank you and your colleagues at MediMabs for successfully making monoclonal antibodies to our immunogen. The timelines you established once we submitted our immunogen were always met and this enabled us to plan our initial screening well. We were very happy that you were able to provide us with five vials each of the fourteen positive clones. The cells you provided were of a high quality as judged by the viability after thawing (viability was greater than 70%). We would also like to thank you for the isotyping data, western blot analysis of the monoclonal antibodies and all advice you provided during this project.