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Vglut1 Mouse Monoclonal Antibody (McKA1)

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Vglut1 Mouse Monoclonal Antibody (McKA1)

SKU:
MM-0016-P

USD 395.00 /100 µg

Clonality: 
Monoclonal
Host: Mouse
Reactivity: Human, Rat
Application: IHC, ICC, ELISA, WB

QTY:

OVERVIEW

Target: 
Vglut1
Target background: 
Glutamate is the major excitatory neurotransmitter in the central nervous system of mammals. Vesicular glutamate transporters (VGluts) are responsible for glutamate transport from the brain into native synaptic vesicles. The level of expression of the three known transporters in the adult brain determines the amount of glutamate that is loaded into vesicles and released.
Target alias: 
Vesicular glutamate transporter 1, VGluT1, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7
Immunogen: 
Full Lenght VGlut1
Specificity: A highly specific and sensitive antibody for VGlut1.The antibody reveals the presence of the 58 kDa VGlut 1 protein on western blot of homogenized cerebral cortex cells lysates. It shows very precise reaction with VGlut1 epitope of human mid-frontal gyrus brain tissue in immunohistochemistry.
Clone ID: 
McKA1
Isotype: 
IgG2a
Preservative: 
None
Format: 
Lyophilized protein G purified in PBS pH7.4
Recommend starting dilution: 
If reconstituted with deionized water in 100 µL: ICC / IHC 1:5,000. Optimal dilution has to be determined by the user
Limitations: 
Research Use Only
Storage: 
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.