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Ubiquitinated H2B Mouse Monoclonal Antibody (NRO3)

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  • Ubiquitinated H2B Mouse Monoclonal Antibody (NRO3)
  • Western blot analysis of Samples from Arabidopsis thaliana 11 days after seed plating using MM-0029-P. (SDS gel 11%). 1: Ladder; 2: Wild type control Col0; 3: bmi1 mutant; 4: ring1 mutant. Same amount of proteins were loaded in each well. Western blot analysis of Samples from Arabidopsis thaliana 11 days after seed plating using MM-0029-P. (SDS gel 11%). 1: Ladder; 2: Wild type control Col0; 3: bmi1 mutant; 4: ring1 mutant. Same amount of proteins were loaded in each well.
  • Western blot analysis of Samples from Arabidopsis thaliana 11 days after seed plating using MM-0029-P. (SDS gel 11%). #1-2-3: Extraction pellets (negative control) - protein of interest should be located in the supernatant if extraction is efficient (#1 corresponds to extration pellet of #4, #2 of #5 and #3 of #6); #4: Total protein extraction (negative control) - Almost totally ineficient method for histone extraction; #5: nuclear protein extraction (Positive control - amount 1X); #6: nuclear protein extraction (Positive control - amount 2X). Western blot analysis of Samples from Arabidopsis thaliana 11 days after seed plating using MM-0029-P. (SDS gel 11%). #1-2-3: Extraction pellets (negative control) - protein of interest should be located in the supernatant if extraction is efficient (#1 corresponds to extration pellet of #4, #2 of #5 and #3 of #6); #4: Total protein extraction (negative control) - Almost totally ineficient method for histone extraction; #5: nuclear protein extraction (Positive control - amount 1X); #6: nuclear protein extraction (Positive control - amount 2X).
  • Western blot analysis of the ubiquitinated histone H2B antibody on whole cell extract from HeLa cells transfected with siRNA directed against the H2B E3 ubiquitin ligase (hBRE1) or LacZ. GAPDH antibody was used as loading control. Western blot analysis of the ubiquitinated histone H2B antibody on whole cell extract from HeLa cells transfected with siRNA directed against the H2B E3 ubiquitin ligase (hBRE1) or LacZ. GAPDH antibody was used as loading control.
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Ubiquitinated H2B Mouse Monoclonal Antibody (NRO3)

SKU:
MM-0029-P

USD 395.00 /100 µg

Clonality: 
Monoclonal
Host: Mouse
Reactivity: Human, Mouse, Drosophila, Arabidopsis
Application: WB, IHC, IF, ChIP

QTY:

OVERVIEW

Target: 
Ubiquitinated H2B
Target background: 
It has been demonstrated that histone modifications are implicated in the regulation of transcription. Recent studies have revealed complex links between H2B ubiquitination and the control of transcription. Some studies suggest that H2B ubiquitination plays a role in transcriptional silencing whereas others suggest a role in transcriptional initiation and elongation. This antibody was raised against a branched peptide corresponding to the conjugation site of ubiquitin on human histone H2B.
Target alias: 
Ubiquitinated Histone H2B, Ubiquitin Histone H2B, H2B, ub histone h2b, ub h2b
Immunogen: 
Branched peptide corresponding to the conjugation site of ubiquitin on human histone H2B
Specificity: The antibody recognizes the K120 ubiquitination of the human histone H2B with no detectable cross-reactivity with either non-ubiquitinated H2B or ubiquitinated histone H2A.
Clone ID: 
NRO3
Isotype: 
IgG2a
Preservative: 
None
Format: 
Lyophilized protein G purified in PBS pH7.4
Recommend starting dilution: 
If reconstituted with deionized water in 50 µL: WB 2-3 µg/ml; ChIP 3-5 µg per sample; IHC: 1:200 – 1:500. Optimal dilution has to be determined by the user.
Limitations: 
Research Use Only
Storage: 
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.