Stromal membrane associated protein-2 (SMAP2, also known as small ArfGAP2) is a GTPase activating protein that plays a role in clathrin-dependent endocytosis. SMAP2 is known to interact with ARF1 and ARF6 (in vitro) and may play a role in clathrin-dependent retrograde transport from early endosomes to the trans-Golgi. It is expressed in multiple foci throughout the cytoplasm and in juxtanuclear structures.
Small ArfGAP2, stromal membrane associated protein-2
Recommend starting dilution:
If reconstituted with deionized water in 100 µl: WB 1:1000; IP native conditions. Optimal dilution has to be determined by the user.
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.