Type IIa receptor protein tyrosine phosphatases (RPTPs) are a group of well-characterized proteins that are involved in axon growth and guidance during neural development. Members of this subfamily, RPTPσ, RPTPδ and LAR contain two cytoplasmic phosphatase domains and extracellular immunoglobulin domains followed by fibronectin type III repeats. RPTPσ is a type I transmembrane protein that has an apparent molecular weight of approximately 80 kDa. It is highly expressed by neurons in developing and adult mammalian nervous system. Knock-out mice for RPTPσ show growth retardation, delayed peripheral nerve development and high mortality rates. The role of RPTPσ in axon guidance during development suggests it could influence axon regeneration after injury in the adult mouse. It has been shown that RPTPσ slows axon regeneration in the adult injured CNS.
R-PTP-S, PTPNU-3, Receptor-type tyrosine-protein phosphatase sigma, R-PTP-sigma, Ptprs
recombinant protein corresponding to the intracellular domain of mouse RPTP .
Lyophilized protein G purified in PBS pH7.4
Recommend starting dilution:
If reconstituted with deionized water in 100 µL: WB 1:2000. Optimal dilution has to be determined by the user.
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.