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Rho-Guanine Exchange Factor Goat Polyclonal Antibody

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  • UPER LEFT: Motor neuron from neuropathologically normal human spinal cord showing normal neuronal expression pattern of RGNEF (1:500 dilution of goat anti-RGNEF, DAB; paraffin embedded human tissue, 5µm section). UPER RIGHT: Motor neuron from ALS affected spinal cord showing skein-like structures of RGNEF (1:500 dilution of goat anti-RGNEF, DAB; paraffin embedded human tissue, 5µm section). LOWER LEFT: Motor neuron from ALS affected spinal cord showing skein-like structures of RGNEF throughout the neuron by confocal microscopy (1:100 dilution of goat anti-RGNEF with fluorescently coupled secondary anti-goat antibody (green); paraffin embedded human tissue, 20µm section; Hoechst (blue) labeled nuclei). LOWER RIGHT: Motor neurons from wild-type C57/BL6 mouse spinal cord (1:100 dilution of goat anti-RGNEF with fluorescently coupled secondary anti-goat antibody (green); paraffin embedded mouse spinal cord tissue; Hoechst (blue) labeled nuclei). UPER LEFT: Motor neuron from neuropathologically normal human spinal cord showing normal neuronal expression pattern of RGNEF (1:500 dilution of goat anti-RGNEF, DAB; paraffin embedded human tissue, 5µm section). UPER RIGHT: Motor neuron from ALS affected spinal cord showing skein-like structures of RGNEF (1:500 dilution of goat anti-RGNEF, DAB; paraffin embedded human tissue, 5µm section). LOWER LEFT: Motor neuron from ALS affected spinal cord showing skein-like structures of RGNEF throughout the neuron by confocal microscopy (1:100 dilution of goat anti-RGNEF with fluorescently coupled secondary anti-goat antibody (green); paraffin embedded human tissue, 20µm section; Hoechst (blue) labeled nuclei). LOWER RIGHT: Motor neurons from wild-type C57/BL6 mouse spinal cord (1:100 dilution of goat anti-RGNEF with fluorescently coupled secondary anti-goat antibody (green); paraffin embedded mouse spinal cord tissue; Hoechst (blue) labeled nuclei).
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Rho-Guanine Exchange Factor Goat Polyclonal Antibody

SKU:
MM-0193-P

USD 355.00 /100 µg

Clonality: 
Polyclonal
Host: Goat
Reactivity: Human, Mouse
Application: IP, IF, IHC

QTY:

OVERVIEW

Target: 
Rho-Guanine Exchange Factor
Target background: 
The human Rho guanine nucleotide exchange factor (RGNEF) is a homolog of mouse p190RhoGEF that has been recently identified as a novel human NFL mRNA binding protein in Amyotrophic Lateral Sclerosis (ALS) affected spinal cords. ALS - also known as Charcot's Disease, Lou Gehrig's disease and Motor Neuron Disease – is a rapidly progressive, and fatal, neuromuscular disease characterized by the gradual degeneration and death of motor neurons in the brain and spinal cord, which lead to progressive paralysis of the muscles.
Target alias: 
RGNEF
Immunogen: 
Peptide within the RNA binding domain of RNEGF
Specificity: The antibody recognizes a sequence on the RNA binding domain of human Rho-Guanine Nucleotide Exchange Factor (RGNEF) isoforms A and B. Competition test were done with a blocking peptide identical to the one used during immunization
Clone ID: 
---
Preservative: 
None
Format: 
Lyophilized immunogen affinity purified in PBS pH7.4
Recommend starting dilution: 
If reconstituted with deionized water in 100 µl: IP: 1ug/ml, IF: 1:100, IHC: 1:100-1:500. Optimal dilution has to be determined by the user.
Limitations: 
Research Use Only
Storage: 
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.