Rho-Guanine Exchange Factor
The human Rho guanine nucleotide exchange factor (RGNEF) is a homolog of mouse p190RhoGEF that has been recently identified as a novel human NFL mRNA binding protein in Amyotrophic Lateral Sclerosis (ALS) affected spinal cords. ALS - also known as Charcot's Disease, Lou Gehrig's disease and Motor Neuron Disease – is a rapidly progressive, and fatal, neuromuscular disease characterized by the gradual degeneration and death of motor neurons in the brain and spinal cord, which lead to progressive paralysis of the muscles.
Peptide within the RNA binding domain of RNEGF
Lyophilized immunogen affinity purified in PBS pH7.4
Recommend starting dilution:
If reconstituted with deionized water in 100 µl: IP: 1ug/ml, IF: 1:100, IHC: 1:100-1:500. Optimal dilution has to be determined by the user.
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.