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Phospho Prion Protein (pPrP Ser43) Rabbit Polyclonal Antibody

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  • Phospho Prion Protein (pPrP Ser43) Rabbit Polyclonal Antibody
  • Antibody specificity was tested by Western blot analysis in non-phosphorylated PrP and Cdk5-phosphorylated PrP, using a monoclonal antibody against the Prion Protein (3F4) and the anti-pPrPS43 (MM-0112). Antibody specificity was tested by Western blot analysis in non-phosphorylated PrP and Cdk5-phosphorylated PrP, using a monoclonal antibody against the Prion Protein (3F4) and the anti-pPrPS43 (MM-0112).
  • Antibody specificity was tested by Western blot analysis using an antibody against the Prion Protein (3F4) of immunoprecipitated PrP and pPrP immunoprecipitated with anti-pPrPS43 (MM-0112) or anti-PrP36-56 R155 polyclonal antibody (which was generated against residues 36–56 of full length WT SHaPrP Antibody specificity was tested by Western blot analysis using an antibody against the Prion Protein (3F4) of immunoprecipitated PrP and pPrP immunoprecipitated with anti-pPrPS43 (MM-0112) or anti-PrP36-56 R155 polyclonal antibody (which was generated against residues 36–56 of full length WT SHaPrP
  • Antibody specificity was tested by Western blot analysis of Cdk5-phosphorylated PrP (pPrP) and mutated pPrP S43A with the 3F4 monoclonal antibody and anti-pPrPS43 (MM-0112). Antibody specificity was tested by Western blot analysis of Cdk5-phosphorylated PrP (pPrP) and mutated pPrP S43A with the 3F4 monoclonal antibody and anti-pPrPS43 (MM-0112).
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Phospho Prion Protein (pPrP Ser43) Rabbit Polyclonal Antibody

SKU:
MM-0112

USD 305.00 /50 µL

Clonality: 
Polyclonal
Host: Rabbit
Reactivity: Mammals
Application: ELISA, IP, IHC, WB

QTY:

OVERVIEW

Target: 
Phospho Prion Protein (pPrP Ser43)
Target background: 
Prion related neurodegenerative diseases, called transmissible spongiform encephalopathies, are observed in many animal species. These diseases involve conversion of normal cellular prion protein (PrPc) into a form that is insoluble and resistant to proteases (PrPSc). The protease resistant form can polymerize into fibrils which accumulate in infected tissues and cause cell death and tissue damage. PrPs have an N-terminal signal sequence and a C-terminal linkage to glycosylphosphatidylinositol anchor. The mature protein is a glycosylated protein that associates with cell membranes. Phosphorylation of PrPC at Ser-43 by Cdk5 promotes proteinase K resistance, prion aggregation, and fibril formation in vitro. In addition, Ser-43 phosphorylation is upregulated in scrapie-infected mouse brain relative to controls. Thus, phosphorylation of Ser-43 may be an important mechanism leading conversion of PrPc to PrPSc and the onset of disease. The antibody was raised against Phospho Prion Protein (pPrP Ser43).
Target alias: 
anti-Phospho Prion Protein, anti-Prion Protein, PrP, ASCR, PrP27-30, PrP33-35C , CD_antigen: CD230
Immunogen: 
Phospho-peptide
Specificity: A highly specific and sensitive antibody against the phosphorylation of the Ser43 of the Prion Protein. The antibody reacts with the Ser43 (G[pSer]PGGNRYPC) of the Phosphorylated Prion Protein.
Clone ID: 
---
Preservative: 
None
Format: 
Lyophilized serum
Recommend starting dilution: 
If reconstituted with deionized water in 50 µL: ELISA (phospho peptide) 1:500,000; E (non-phospho peptide) 1:25,000 WB 1:100; IP 1:100; IHC 1:200-1:1000. Optimal dilution has to be determined by the user
Limitations: 
Research Use Only
Storage: 
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.