Protein unc-13 homolog D (Munc13-4) is involved in cytotoxic granule exocytosis in lymphocytes. It regulates the assembly of recycling of late endosomal structures which leads to the formation of an endosomal exocytic compartment. It also regulates Ca2+-dependent secretory lysosome exocytosis in mast cells. Munc13-4 is highly expressed in the spleen, thymus and leukocytes and to a lesser extent in the lung and placenta, brain, heart, skeletal muscle and kidney. Defects in Munc13-4 are the cause of familial HL type 3 (FHL3).
Protein unc-13 homolog D, hMunc13-4, UNC13D, FHL3, HLH3, HPLH3, Munc13-4, unc-13 homolog D
Recommend starting dilution:
If reconstituted with deionized water in 50 µl: WB: 1:2000; IP: native conditions. Optimal dilution has to be determined by the user.
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.