Free shipping on antibody orders delivered to Canada

Misfolded SOD1 Mouse Monoclonal Antibody (A5C3)

  • Home
  • /
  • Misfolded SOD1 Mouse Monoclonal Antibody (A5C3)
  • The spinal cord sections of transgenic mice SOD1G93A show the detection of misfolded SOD1 in the motor neurons. This immunodetection does not appear in the transgenic mice that reproduce normal human SOD1 (Tg-hWTov Sc). MM-0070-3-P: 1:1000 The spinal cord sections of transgenic mice SOD1G93A show the detection of misfolded SOD1 in the motor neurons. This immunodetection does not appear in the transgenic mice that reproduce normal human SOD1 (Tg-hWTov Sc). MM-0070-3-P: 1:1000
  • Alt

Misfolded SOD1 Mouse Monoclonal Antibody (A5C3)

SKU:
MM-0070-3-P

USD 495.00 /200 µL

Clonality: 
Monoclonal
Host: Mouse
Reactivity: Human
Application: WB, IP, IF

QTY:

OVERVIEW

Target: 
Misfolded SOD1
Target background: 
Superoxide dismutase 1 (SOD1) is a soluble cytoplasmic and mitochondrial intermembrane space protein. SOD1 binds copper and zinc ions and is one of three isozymes responsible for destroying free superoxide radicals in the body. Mutations in SOD1 cause familial amyotrophic lateral sclerosis type 1 (ALS1). These mutations have been linked to accumulation of harmful superoxide radicals, promotion of apoptosis, formation of aggregates of misfolded superoxide dismutase which are toxic and the continued stimulation of nerve cells that causes them to burn out and die.
Target alias: 
Superoxide dismutase [Cu-Zn], Superoxide dismutase 1, hSOD1, SOD1
Immunogen: 
Recombinant human SOD1 G93A APO
Specificity: The antibody recognizes misfolded forms of mutant human SOD1 protein. IF: G93A, G85R, and G37R; WB (denatured): G93A, G127X and G37R; WB (native): G37R; IP: G93A, G127X and G37R.
Clone ID: 
A5C3
Isotype: 
IgG1
Preservative: 
None
Format: 
Lyophilized protein G purified in PBS pH7.4
Recommend starting dilution: 
If reconstituted with deionized water in 200 µL: IF 1:50 to 1:500; WB 1:250; IP 5 µL / 40 µL (Protein G beads / 300 µg of protein lysate). Optimal dilution must be determined by the user.
Limitations: 
Research Use Only
Storage: 
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.