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Leu-enkephalin Mouse Monoclonal Antibody (NOC1)

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  • Leu-enkephalin Mouse Monoclonal Antibody (NOC1)
  • Cat spinal cord immunostained with NOC1 (Anti-LeuEnkephalin 1018) Cat spinal cord immunostained with NOC1 (Anti-LeuEnkephalin 1018)
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Leu-enkephalin Mouse Monoclonal Antibody (NOC1)

SKU:
1018

USD 355.00 /0.5 mL

Clonality: 
Monoclonal
Host: Mouse
Reactivity: Rat, Human
Application: IHC, ICC, RIA

QTY:

OVERVIEW

Target: 
Leu-enkephalin
Target background: 
Enkephalins (penta-peptides) have been discovered as regulators of nerve impulses involving pain in the brain. It has been shown that these peptides act as natural analgesics (pain-killers) and their action mimics that of morphine and other opiates. At present, it is thought that the morphine-like effects are due to aromatic side chains on phenylanine and tyrosine which mimic a similar structure on morphine. Research suggests that it does not make much difference whether the enkephalin contains methionine or leucine at the acid end of the peptide for its action.
Target alias: 
Enkephalin, endorphins, endorphin, Met5-enkephalin, Leu5-enkephalin, Met-enkephalin, Leu-enkephalin, anti-Enkephalin, anti-endorphins, anti-endorphin, anti-Met5-enkephalin, anti-Leu5-enkephalin, anti-Met-enkephalin, anti-Leu-enkephalin
Immunogen: 
pentapeptide ( Tyr-Gly-Gly-Phe-Leu)
Specificity: A sensitive antibody against enkephalinergic fibrilar networks in the globus pallidus, N. accumbens, septum, hypothalamus, thalamus, amygdala, N. interstitialis of the stria terminalis and brain stem nuclei. This antibody does not distinguish between Met5-enkephalin and Leu5-enkephalin by radioimmunoassay or by immunocytochemistry. It is recommended for localizing enkephalinergic sites by immunohistochemistry. It does not bind to beta-endorphin or dynorphin-containing areas. It exhibits approximately. 40% cross reactivity with c-terminal extended Met-enkephalin hexapeptides and 7% cross reactivity with the extended heptapeptide (-Arg-Phe-0H), but does not recognize other endogenous peptides. All wellestablished enkephalin immunoreactive sites are recognized by this antibody in immunohistochemistry (IHC) and electron microscopy immunocytochemistry (ICC). Specific for: rat and human
Clone ID: 
NOC1
Isotype: 
IgG1
Preservative: 
0.05% thimerosal
Storage: 
Lyophilized antibody can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20ºC) or for long-term storage (-80ºC). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Format: 
Lyophilized tissue culture supernatant
Recommend starting dilution: 
If reconstituted in 0.5 mL: IHC 1:100 - 1:400. Optimal dilution has to be determined by the user.
Limitations: 
Research Use Only