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GAPDH Mouse Monoclonal Antibody (GA1R)

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  • GAPDH Mouse Monoclonal Antibody (GA1R)
  • Western blot analysis of GAPDH expression. LEFT: 1:2,000 (0.5μg/mL) Ab dilution used in WB of 5μg/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6). RIGHT: WB from BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3). Western blot analysis of GAPDH expression. LEFT: 1:2,000 (0.5μg/mL) Ab dilution used in WB of 5μg/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6). RIGHT: WB from BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3).
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GAPDH Mouse Monoclonal Antibody (GA1R)

SKU:
MM-0163-P

USD 205.00 /100 µg

Clonality: 
Monoclonal
Host: Mouse
Reactivity: Human, Mouse, Rat, rabbit, Chicken, Hamster, BL-21 bacteria, Sf9 insect, and Saccharomyces cerevisiae (yeast)
Application: ELISA, WB, Dot, IHC

QTY:

OVERVIEW

Target: 
GAPDH
Target background: 
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a 37KDa glycolytic enzyme constitutively and stably expressed in almost all tissues. This enzyme catalyzes the sixth step of glycolysis by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Proteins such as GAPDH involved in maintenance of basic cellular function are often referred to as housekeeping proteins. Housekeeping proteins are frequently used as loading controls for western blots and protein normalization.
Target alias: 
Glyceraldehyde 3-phosphate dehydrogenase
Specificity: The antibody recognizes native and denatured forms of GAPDH.
Clone ID: 
GA1R
Isotype: 
IgG1
Preservative: 
None
Format: 
Lyophilized protein G purified in PBS pH7.4
Recommend starting dilution: 
If reconstituted with deionized water in 100 µl: WB 1:1000-2,000, IHC 1:500-2000. Optimal dilution has to be determined by the user.
Limitations: 
Research Use Only
Storage: 
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.