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FGFR2c Human Recombinant Antibody

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  • FGFR2c Human Recombinant Antibody
  • Capture ELISA of FGFR2c: As validation of the antibody, 1ug/mL of FGFR2c antigen  (Human or Mouse) or control protein (Fc or BSA) were adsorbed onto maxisorp plates, blocked with PBS + 0.5% BSA (PB), and washed 3 times with PBS + 0.05% Tween-20 (PT); 1 ug /ml anti-FGFR2c Fab antibody in PBS for 30 min, shaking RT, washed 6 times with PT.  Anti-human kappa-HRP (1:5000 in PB) was incubated for 30 min, shaking RT, washed 6 times with PT. Colorimetric HRP reagents allow for absorbance readings at 450 nm. Capture ELISA of FGFR2c: As validation of the antibody, 1ug/mL of FGFR2c antigen (Human or Mouse) or control protein (Fc or BSA) were adsorbed onto maxisorp plates, blocked with PBS + 0.5% BSA (PB), and washed 3 times with PBS + 0.05% Tween-20 (PT); 1 ug /ml anti-FGFR2c Fab antibody in PBS for 30 min, shaking RT, washed 6 times with PT. Anti-human kappa-HRP (1:5000 in PB) was incubated for 30 min, shaking RT, washed 6 times with PT. Colorimetric HRP reagents allow for absorbance readings at 450 nm.
  • Isoform-specificity of FGFR2c: 1µg/mL of FGFR family of proteins or control proteins (Fc and BSA) were adsorbed onto maxisorp plates, blocked with PBS + 0.5% BSA (PB), and washed 3 times with PBS + 0.05% Tween-20 (PT); 0.2umg /ml anti-FGFR2c hIgG1 antibody in PBS for 30 min, shaking RT, washed 6 times with PT.  Anti-human kappa-HRP (1:5000 in PB) was incubated for 30 min, shaking RT, washed 6 times with PT. Colorimetric HRP reagents allow for absorbance readings at 450 nm Isoform-specificity of FGFR2c: 1µg/mL of FGFR family of proteins or control proteins (Fc and BSA) were adsorbed onto maxisorp plates, blocked with PBS + 0.5% BSA (PB), and washed 3 times with PBS + 0.05% Tween-20 (PT); 0.2umg /ml anti-FGFR2c hIgG1 antibody in PBS for 30 min, shaking RT, washed 6 times with PT. Anti-human kappa-HRP (1:5000 in PB) was incubated for 30 min, shaking RT, washed 6 times with PT. Colorimetric HRP reagents allow for absorbance readings at 450 nm
  • Flow Cytometry of FGFR2c: H1581 cells were washed in PBS. 5x105 cells were incubated with or without 0.1 ug of anti-FGFR2c hIgG1, or scIgG1, or schmIgG2a anti-FGFR2c antibodies in 100 uL PBS + 0.1% BSA (PB) for 30 min on ice, then washed twice with cold PB. Samples were then incubated with Goat anti-Human Fab-488 (Jackson ImmunoResearch, diluted 1:1000 PB) for 15-30 min, washed twice with cold PBS and fixed with 2% paraformaldehyde and filtered prior to reading on a FACS machine. Flow Cytometry of FGFR2c: H1581 cells were washed in PBS. 5x105 cells were incubated with or without 0.1 ug of anti-FGFR2c hIgG1, or scIgG1, or schmIgG2a anti-FGFR2c antibodies in 100 uL PBS + 0.1% BSA (PB) for 30 min on ice, then washed twice with cold PB. Samples were then incubated with Goat anti-Human Fab-488 (Jackson ImmunoResearch, diluted 1:1000 PB) for 15-30 min, washed twice with cold PBS and fixed with 2% paraformaldehyde and filtered prior to reading on a FACS machine.
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FGFR2c Human Recombinant Antibody

SKU:
MM-0309

USD 485.00 /100 µg

Clonality: 
Monoclonal
Host: Human; made using CCAB phage display library
Reactivity: Human; cross-reacts with Mouse
Application: Flow Cytometry

QTY:

OVERVIEW

Target: 
Human FGFR2c
Target background: 
(FGFR2c) This member of the Fibroblast Growth Factor Receptor (FGFR) family consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. [provided by RefSeq, Jan 2009]
Target alias: 
BEK, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25
Immunogen: 
ECD of recombinant FGFR2c; aa: R22-E377
Specificity: This antibody recognizes the ECD of FGFR2c (aa: R22-E377) and does not recognize FGFR1c, 2b, 3b, 3c, 4
Clone ID: 
CR - 010 (generated by CCAB)
Isotype: 
hIgG1; also available as mIgG1, scIgG1, schmIgG2a
Preservative: 
None
Format: 
Lyophilized protein A purified in PBS pH7.4
Recommend starting dilution: 
Reconstitute with deionized water. Optimal dilution has to be determined by the user.
Limitations: 
Research Use Only; Manufactured by CCAB under a license using Life Technologies proprietary cell lines.
Storage: 
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.