Estrogen Sulfotransferase (EST)
Estrogen sulfotransferase (EST) is a cytosolic enzyme that catalyzes specific sulfonation with a high affinity for estrogens. Because EST is involved in estrone and estradiol metabolism, its expression in the male reproductive tract determines where, along the tract, an estrogenic environment predominates. EST may also be involved in sperm physiology during the epididymal transit.
anti-estrogen sulfotransferase, anti-est , Sulfotransferase 1E1 , ST1E1 , Sulfotransferase, estrogen-preferring , SULT1E1
Full length recombinant bovine EST protein
Lyophilized immunogen affinity purified in PBS pH7.4
Recommend starting dilution:
If reconstituted with deionized water in 100µL: WB 1 μg/ml; IHC 5 μg/ml; IF 10 μg/ml. Optimal dilution has to be determined by the user.
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.