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E-cadherin Human Recombinant Antibody

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  • E-cadherin Human Recombinant Antibody
  • Capture ELISA of E-Cadherin: As validation of the antibody, 1ug/mL of E-Cadherin antigen or control protein (Fc or BSA) were adsorbed onto maxisorp plates, blocked with PBS + 0.5% BSA (PB), and washed 3 times with PBS + 0.05% Tween-20 (PT); 0.2ug /ml anti-E-Cadherin hIgG1 antibody in PBS for 30 min, shaking RT, washed 6 times with PT.  Anti-human kappa-HRP (1:5000 in PB) was incubated for 30 min, shaking RT, washed 6 times with PT. Colorimetric HRP reagents allow for absorbance readings at 450 nm. Capture ELISA of E-Cadherin: As validation of the antibody, 1ug/mL of E-Cadherin antigen or control protein (Fc or BSA) were adsorbed onto maxisorp plates, blocked with PBS + 0.5% BSA (PB), and washed 3 times with PBS + 0.05% Tween-20 (PT); 0.2ug /ml anti-E-Cadherin hIgG1 antibody in PBS for 30 min, shaking RT, washed 6 times with PT. Anti-human kappa-HRP (1:5000 in PB) was incubated for 30 min, shaking RT, washed 6 times with PT. Colorimetric HRP reagents allow for absorbance readings at 450 nm.
  • Flow Cytometry of E-Cadherin: MCF7 (E-Cadherin positive) cells expressing surface E-cadherin were collected using a mild EDTA solution and washed in PBS.  5x105 cells were incubated with 1ug of anti-E-Cadherin Fab antibody in 100 ul  PBS + 0.1% BSA for 30 min on ice, then washed twice with cold PBS + 0.1 % BSA. Sample was then incubated with Goat anti-Human Fab-488 (Jackson ImmunoResearch, diluted with 1:1000 PBS + 0.1% BSA) for 15-30 min, washed twice with cold PBS + 0.1% BSA and fixed with 2% paraformaldehyde and filtered prior to reading on a FACS machine. Flow Cytometry of E-Cadherin: MCF7 (E-Cadherin positive) cells expressing surface E-cadherin were collected using a mild EDTA solution and washed in PBS. 5x105 cells were incubated with 1ug of anti-E-Cadherin Fab antibody in 100 ul PBS + 0.1% BSA for 30 min on ice, then washed twice with cold PBS + 0.1 % BSA. Sample was then incubated with Goat anti-Human Fab-488 (Jackson ImmunoResearch, diluted with 1:1000 PBS + 0.1% BSA) for 15-30 min, washed twice with cold PBS + 0.1% BSA and fixed with 2% paraformaldehyde and filtered prior to reading on a FACS machine.
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E-cadherin Human Recombinant Antibody

SKU:
MM-0306

USD 310.00 /100 µg

Clonality: 
Monoclonal
Host: Human; made using CCAB phage display library
Reactivity: Human
Application: Flow Cytometry

QTY:

OVERVIEW

Target: 
Human E-cadherin
Target background: 
This gene includes a classical cadherin of the cadherin superfamily. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, transmembrane region and a highly conserved cytoplasmic tail. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. [provided by RefSeq]
Target alias: 
CDH1, CD324, UVO, uvomorulin
Immunogen: 
ECD of recombinant E-cadherin: aa: D155-I707
Specificity: This antibody recognizes the ECD of E-cadherin (aa: D155-I707)
Clone ID: 
CR - 007 (generated by CCAB)
Isotype: 
hIgG1; also available as mIgG1, scIgG1, schmIgG2a
Preservative: 
None
Format: 
Lyophilized protein A purified in PBS pH7.4
Recommend starting dilution: 
Reconstitute with deionized water. Optimal dilution has to be determined by the user.
Limitations: 
Research Use Only; Manufactured by CCAB under a license using Life Technologies proprietary cell lines.
Storage: 
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.