ARNO / Cytohesin-2 acts as guanine-nucleotide exchange factor (GEF) for ADP-ribosylation factor such as ARF1, ARF3 and ARF6. Both isoforms (1 and 2) of ARNO / Cytohesin-2 are located in the cytoplasm and in cell membrane after being recruited through their association with ARL4A, ARL4C and ARL4D
Recommend starting dilution:
If reconstituted with deionized water in 100 µL: WB: 1:2000; IHC: 1:100-1:250. Optimal dilution has to be determined by the user.
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.