Tubulin cleaved by caspase-6
Tubulin is the major constituent of microtubules. Microtubules are assembled from dimers of α- and β-tubulin. α-Tubulin cleaved by caspase-6 can be found in neurons, neurofibrillary tangles, neutrophil threads, and neuritic plaques in Alzheimer disease. α-Tubulin has been found to associate with the axonal microtubule-associated protein Tau, indicating that the cleavage of the functional α-Tubulin C-terminal domain by caspase-6 could alter the state of microtubules in neurons.
α-Tubulin cleaved by casp 6
Recommend starting dilution:
If reconstituted with deionized water in 50 µl: WB 1:1000; IHC 1:1000. Optimal dilution has to be determined by the user.
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.